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ExoView外泌体全面表征试剂盒-NanoView

ExoView外泌体全面表征试剂盒-NanoView

简要描述:ExoView外泌体全面表征试剂盒-NanoView拥有异性捕获,对阳性外泌体计数,单个外泌体蛋白共定位分析,无需纯化,粒径分辨率高和可检测外泌体内容物等点。将助力于外泌体检测,外泌体粒径以及外泌体计数,实现外泌体计数、粒径、蛋白表达、蛋白共定位次完成。

产品型号:

所属分类:单分子生物学

更新时间:2021-07-09

厂商性质:生产厂家

详情介绍
品牌其他品牌价格区间面议
产地类别进口应用领域医疗卫生,生物产业

ExoView外泌体全面表征试剂盒

外泌体计数、粒径、蛋白表达、蛋白共定位次完成

检测样本类型

对细胞培养上清、血浆、血清、尿液、脑脊液、唾液等生物样本中的外泌体直接进行分析

捕获抗体种类

anti-CD81, anti-CD9, anti-CD63, 同型IgG对照;可自定义

单次上样体积

35 µl稀释样本

重复检测数目

3复孔

荧光抗体种类

CD9(Blue)/ CD81(Green)/ CD63(Red)

 

实验原理

① 35 μL外泌体样品滴加在芯片上孵育;

② 预包被的抗体异结合外泌体表面蛋白以捕获外泌体;

③ 再使用荧光抗体异性标记需要表征的标记物;

④ 后用ExoView R100检测外泌体粒径、计数、蛋白表达(CD9,CD81,CD63等)及共定位。

 

检测流程


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产品类别

产品货号产品名称
EV-TETRA-C人外泌体检测试剂盒
EV-TETRA-P人血浆外泌体检测试剂盒
EV-TETRA-M2鼠外泌体检测试剂盒
EV-TETRA-C-CAR人外泌体内容物检测试剂盒
EV-TETRA-P-CAR人血浆外泌体内容物检测试剂盒
EV-TC-FLEX自由捕获人外泌体检测试剂盒
EV-TP-FLEX自由捕获人血浆外泌体检测试剂盒
EV-TC-FLEX-CAR自由捕获人外泌体内容物检测试剂盒
EV-TP-FLEX-CAR自由捕获人血浆外泌体内容物检测试剂盒
EV-TM-FLEX自由捕获鼠外泌体检测试剂盒
EV-TM-FLEX-CAR自由捕获鼠外泌体内容物检测试剂盒
EV-FLEX-2自由捕获外泌体检测试剂盒
EV-FLEX-2 -CAR自由捕获外泌体内容物检测试剂盒
EV-CTETRA-1/2/3人外泌体检测试剂盒+1/2/3个自定义捕获抗体
EV-CTETRA-1/2/3-CAR人外泌体内容物检测试剂盒+1/2/3个自定义捕获抗体
EV-CUST-1/2/3/4/5/6自定义1/2/3/4/5/6抗体捕获外泌体检测试剂盒
EV-CUST-1/2/3/4/5/6-CAR自定义1/2/3/4/5/6抗体捕获外泌体内容物检测试剂盒

 

试剂盒点

异性捕获

芯片上可包被多达6种捕获抗体,异性捕获含定蛋白标记物的外泌体。

 

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对阳性外泌体计数

芯片捕获外泌体后,可通过SP-IRIS技术直接检测样品中外泌体的数量。

 

 

单个外泌体蛋白共定位分析

检测每个外泌体的荧光信号并进行统计,可获得荧光共定位信息,用于分析样品中不同表型外泌体的比例(如右图所示)。

 

 

无需纯化

 

使用抗体捕获模式,防止样品中杂质影响结果,可直接检测血液、尿液和细胞培养液中的外泌体,未纯化样品的测量结果与纯化后基本致(如右图所示)。

 

 

粒径分辨率高

 

高精度SP-IRIS技术,可检测≥50 nm的外泌体,测量结果与电子显微镜检测结果基本致,并统计生成外泌体的粒径分布结果(如右图所示)。

 

 

可检测外泌体内容物

 

试剂盒配套相应的穿膜剂,可穿透外泌体并对外泌体内容物进行染色并检测,未穿膜时只能检测到跨膜蛋白CD9的荧光信号,穿膜后即可检测到外泌体内容物Syntenin的表达(如右图所示)。

 

 



测试数据

外泌体荧光数量统计

 

外泌体粒径检测

 

外泌体内容物检测

 

荧光强度与粒径关系

 

荧光共定位分析

发表文章

• Andras Saftics.(2021) Data evaluation for surface-sensitive label-free methods to obtain real-time kinetic and structural information of thin films: A practical review with related software packages. Advances in Colloid and Interface Science

• Kyoung-Won Ko.(2021) Integrated Bioactive Scaffold with Polydeoxyribonucleotide and Stem-Cell-Derived Extracellular Vesicles for Kidney Regeneration. ACS Nano

• Tanina Arab. (2021) Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms. Journal of Extracellular Vesicles

• Niaz Z.Khan.(2021) Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential. Brain, Behavior, and Immunity

• Dario Brambilla. (2021) EV Separation: Release of Intact Extracellular Vesicles Immunocaptured on Magnetic Particles. Analytical Chemistry

• Enkhtuya Radna. (2021) Extracellular vesicle mediated feto-maternal HMGB1 signaling induces preterm birth. Lab on a Chip

• Li, M., Soder. (2021) WJMSC‐derived small extracellular vesicle enhance T cell suppression through PD‐L1. Journal of Extracellular Vesicles

• Crescitelli, R. (2021) Isolation and characterization of extracellular vesicle subpopulations from tissues. Nature protocols

• Berger, A. (2021). Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula. Nanoscale

• Vidal, M. (2020) Exosomes and GPI-anchored proteins: Judicious pairs for investigating biomarkers from body fluids. Advanced drug delivery reviews

• K Cho, H Kook.(2020)Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy. Journal of Extracellular Vesicles. 

• Maximillian A. Rogers.(2020)Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis. Cell Biology

• Annette M. Marleau.(2020)Targeting tumor-derived exosomes using a lectin affinity hemofiltration device. Cancer Research

• Alessandro Gori.(2020)Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis. Journal of Extracellular Vesicles

• Rossella Crescitelli.(2020)Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. Journal of Extracellular Vesicles

• Maria S. Panagopoulou.(2020) Phenotypic analysis of extracellular vesicles:  a review on the applications of fluorescence. Journal of Extracellular Vesicles.

• WeiYan.(2020) Immune Cell-Derived Exosomes in the Cancer-Immunity Cycle. Trends in Cancer

• Daniel Bachurski. (2019) Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation. Cell.

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