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NEWS INFORMATION生物通主办的“2021生命科学十·大创新产品”于2022年1月正式揭晓,Quantum Design中国·新引进的NanoView R200全自动外泌体荧光检测分析系统,凭借其*的设计理念及·越的性能,荣获“2021生命科学十·大创新产品”!
“生命科学十·大创新产品”是生物通历经10余年打造的生命科学科学仪器行业·进、创新的奖项之。至今已举办十四届。本次评选共有28台仪器参选,经过评审家严格筛选以及广大科研用户的投票评比,终评选出10·大创新产品。该奖项致力于让大家了解生命科学发展的新方向,也希望新颖的产品能激发研究者的灵感,推动科研进程,助力科研学者早日攀上科学研究的高峰!
全自动外泌体荧光检测分析系统ExoView是美国NanoView Biosciences于2018年研究推出的,该系统经推出,便引起了外泌体域科研工作者的广泛关注。仅两年已有100多个实验室采用该技术,发表文献近百篇,刊载在Cell, Cell Biology, Journal of Extracellular Vesicles等期刊。ExoView的精准表征,为科学家提供了种无须纯化即可直接对血浆、血清、尿液、培养基中的外泌体进行表征分析的新方法。能够提供单个外泌体水平下的全·方位外泌体表征信息(包括粒径大小、分布、计数、携带蛋白表达与生物标志物(CD9,CD81,CD63等)共定位等),以及多层次和全方面的外泌体测量解决方案。能够帮助科学家更深入地了解外泌体与疾病之间的关系,助力疾病诊断和新药开发。
ExoView® R200全自动外泌体荧光检测分析系统势包括:
• 异性捕获:芯片上可包被多达6 种捕获抗体,异性捕获含定蛋白标记物的外泌体。
• 对阳性外泌体计数:芯片捕获外泌体后,可通过单粒子干涉反射成像传感器(SP-IRIS)技术直接检测样品中外泌体的数量。
• 单个外泌体蛋白共定位分析:检测每个外泌体的荧光信号并进行统计,可获得荧光共定位信息,用于分析样品中不同表型外泌体的比例。
• 无需纯化:使用抗体捕获模式,防止样品中杂质影响结果,可直接检测血液、尿液和细胞培养液中的外泌体,未纯化样品的测量结果与纯化后基本致。
• 粒径分辨率高:高精度SP-IRIS技术,可检测≥50 nm的外泌体,测量结果与电子显微镜检测结果基本致,并统计生成外泌体的粒径分布结果。
• 可检测外泌体内容物:试剂盒配套相应的穿膜剂,可穿透外泌体并对外泌体内容物进行染色并检测,如下图所示,Non-permeabilized时只能检测到跨膜蛋白CD9的荧光信号, Permeabilized后即可检测到外泌体内容物(如PD-L1、P-tau等)的表达。
部分应用单位:
部分发表文献:
• Andras Saftics.(2021) Data evaluation for surface-sensitive label-free methods to obtain real-time kinetic and structural information of thin films: A practical review with related software packages. Advances in Colloid and Interface Science.
• Kyoung-Won Ko.(2021) Integrated Bioactive Scaffold with Polydeoxyribonucleotide and Stem-Cell-Derived Extracellular Vesicles for Kidney Regeneration. ACS Nano.
• Tanina Arab. (2021) Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms. Journal of Extracellular Vesicles.
• Niaz Z.Khan.(2021) Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential. Brain, Behavior, and Immunity.
• Dario Brambilla. (2021) EV Separation: Release of Intact Extracellular Vesicles Immunocaptured on Magnetic Particles. Analytical Chemistry.
• Enkhtuya Radna. (2021) Extracellular vesicle mediated feto-maternal HMGB1 signaling induces preterm birth. Lab on a Chip.
• Li, M., Soder. (2021) WJMSC‐derived small extracellular vesicle enhance T cell suppression through PD‐L1. Journal of Extracellular Vesicles.
• Crescitelli, R. (2021) Isolation and characterization of extracellular vesicle subpopulations from tissues. Nature protocols.
• Berger, A. (2021). Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula. Nanoscale.
• Vidal, M. (2020) Exosomes and GPI-anchored proteins: Judicious pairs for investigating biomarkers from body fluids. Advanced drug delivery reviews.
• K Cho, H Kook.(2020)Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy. Journal of Extracellular Vesicles.
• Maximillian A. Rogers.(2020)Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis. Cell Biology.
• Annette M. Marleau.(2020)Targeting tumor-derived exosomes using a lectin affinity hemofiltration device. Cancer Research.
• Alessandro Gori.(2020)Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis. Journal of Extracellular Vesicles.
• Rossella Crescitelli.(2020)Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. Journal of Extracellular Vesicles.
• Maria S. Panagopoulou.(2020) Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence. Journal of Extracellular Vesicles.
• WeiYan.(2020) Immune Cell-Derived Exosomes in the Cancer-Immunity Cycle. Trends in Cancer.
• Daniel Bachurski. (2019) Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation. Cell.
为了更好的服务中国客户,Quantum Design中国子公司在北京建立了业的客户服务中心,正式推出业的全·方位外泌体表征测试服务,您只需要少量样品即可获得全·方位的外泌体表征数据。
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1、全自动外泌体荧光检测分析系统
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